UTSW Microarray Core Facility

microRNA microarray slides and service

MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNA molecules that are estimated to regulate at least 30% of all protein encoding genes. Currently, more than 2,900 miRNAs have been annotated from a wide range of organisms, including plants, worms, flies, mammals, and viruses. To date, miRNAs have been implicated in development, differentiation, apoptosis, fat metabolism, viral infection, and cancer. Recent studies suggest that miRNAs might be used in future therapeutic and diagnostic applications. Evaluation of the global expression of miRNAs will likely identify regulation points for many different biological processes.

Price

  • microRNA microarray slide: $60 per slide
  • Post-processing: $10/slide
  • microRNA extraction: $50 per sample
  • microRNA probe labeling service: $75 per sample
  • hibridization and scanning: $65 per slides (two samples)

We have purchased the mirVana miRNA Probe Set which is a collection of amine-modified DNA oligonucleotides targeting a comprehensive selection of human, mouse, and rat miRNAs from miRBase (total of 386 miRNA probes). The probes in the mirVana miRNA Probe Set are 42-46 nucleotides (nt) long and consist of an 18-24 nt segment that targets a specific known human, mouse, or rat miRNA, plus spacer and attachment sequences for coupling to the surface of the microarray slide. The probes are synthesized with an amine-modification for compatibility with both epoxy and aldehyde slide surface attachment chemistries. The oligo set was printed on SCHOTT Nexterion® Slide E microarray slides with 6 replicated spots for each probe.

miRNA probe list

For detailed info of microRNA probe set, go to http://www.ambion.com/techlib/resources/miRNA/index.html

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