Sample Submission
Policies and Procedures
Sample submission Procedures
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Check our website or call us to find out the prices for chips/slides and
services.
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Order online. Click on the “online ordering” button in the web front page.
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Bring your samples and
a gel picture showing RNA quality ( Or order our bioanalyzer service).
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You will be notified when your order is done.
RNA Sample requirements
Depending on which service, Affymetrix,Illumina, spotted arrays require at
least 2ug, 500ng and 1ug respectively, in RNase-free water, with concentration
greater than 100ng/ul.Bioanalyzer or OD check needs about 50ng in 2ul. Extra
amount is always helpful, especially when first trial is failed, although the
chance is small.
The concentration of your total RNA must be at least 0.1 ug/ul, and cRNA
concentration must be greater than 0.65 ug/ul in RNase-free water.
Your total RNA or cDNA must be in good quality: 260/280
≥1.7, 260/230 ≥
1.6, RNA Integrity Number (RIN) ≥6.5
Storage of RNA Samples
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The core may store your total RNA samples for up to 3 months. If
you want us to keep them, please indicate so when you place your online order. Please note that we are not a long-term storage facility. If
you wish to retrieve your samples, you must do so within the 3 month time limit
of placing your order.
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The core may store your cRNA/aRNA for
up to one month. If you later wish
to use them to do replicates of arrays or Affy B/C subtypes (using cRNA/aRNA
could save most of the costs), please do so within this one month time period,
or retrieve them to store in your own lab freezer.
Information Specific to Spotted Arrays
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When you order a spotted array full service, the convention used is to label
the experimental sample with Cy 5 and the control sample with Cy 3. If
you wish to reverse the dyes, you must indicate this in the comment section of
your online order.