Microarray Core Facility

illumina Gene Expression and Methylation

illumina whole genome gene expression arrays.

RNA Sample Requirements for Illumina Gene Expression Array

Require total RNA at least 500ng, and cRNA at least 1500ng in RNase-free water.
The concentration of total RNA must be at least 100ng/ul, and cRNA concentration must be greater than 150ng/ul.
The total RNA and cRNA must be in good quality: 260/280>=1.7, 260/230>=1.4, RNA Integrity Number >=7.0.