Microarray Core Facility

Autoantibody Profiling Service for IgG subclass IgG2 and IgG4

IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG2 and IgG4 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks.
$75 per sample (chip not included), maximum 15 samples per chip.

In-Campus Service Price : $75.00  (price may vary for off-campus customers)
In Stock

Please select products and services from the following list:

In-Campus-Price Product
$500.00 Autoantigen Microarray Panel I
Autoantigen array panel I contains 95 autoantigens and 8 internal control antigens, including nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, etc. The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control. Autoantigen array panel I contains 95 autoantigens and 8 internal control antigens, including nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, etc, as listed below:
$500.00 Autoantigen Microarray Panel II
Autoantigen array panel II contains 92 autoantigens and 8 internal control antigens. The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.
$500.00 Autoantigen Microarray Panel III
Autoantigen array panel III contains more than 90 autoantigens and 8 internal control antigens. The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.
$500.00 Autoantigen Microarray Panel IV
Autoantigen panel IV contains 61 antigens related with Pathogens, allergens, cancer specific proteins, myositis antigens and others. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.
$750.00 Autoantigen Microarray Super Panel (128 antigen panel)
Autoantigen array super panel I contains 128 autoantigens and various internal controls. The antuantigens in this panel include most of nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, as well as some novel autoantigen identified from serum and tissues.
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