Microarray Core Facility

Autoantigen Microarray Panel IV

Autoantigen panel IV contains 61 antigens related with Pathogens, allergens, cancer specific proteins, myositis antigens and others.



Pathogen AntigensMyositis Antigens
CMV-G ANTIGEN3-hydroxy-3-methylglutaryl-coenzyme A reductase
CMV-M ANTIGENAminoacyl-tTNA Synthetase
CMV EXT-2 ANTIGENAsparaginyl-tTNA Synthetase
CMV GRADE III ANTIGENGlycyl-tTNA Synthetase
HEPATITIS A ANTIGENLysyl-tTNA Synthetase
HAV CONCENTRATEPhenylalanyl-tTNA Synthetase 2
HSV-1 ANTIGENHuman Cytosolic 5'-nucleotidase 1A
HSV-2 ANTIGENHuman glutaminyl-tTNA Synthetase
RUBEOLA ANTIGENHuman MORC family CW-type zinc finger 3 (MORC3)
RSV ANTIGENHuman signal recognition particle 14kDa
ROTAVIRUS SA-11 ATNIGENHuman SUMO1 activating enzyme subunit 1(SAE1)
RUBELLA VIRUS GRADE III ANTIGENHuman tryptophanyl-tTNA Synthetase(WARS)
RUBELLA VIRUS GRADE IV ANTIGENHuman tyrosyl-tTNA Synthetase(YARS)
RUBELLA GRADE IV ANTIGEN W/PBSHuman ubiquitin-like modifier activating anzyme 2(UBA2)
RSVP ANTIGEN
TOXOPLASMA IgG ANTIGENCancer Antigens
VZV ANTIGENNY-ESO-1
VZV GRADE II ANTIGENHuman Prostatic Acid Phosphatase
HUMAN AZUROCDINHuman Prostate Specific Membrane Antigen
MAGEA3
Allergen AntigensFOLH1 protein
House DustPSA protein
Dog Dander, Mixed-Breed_Canis familiarisCA 125 protein
Dog Epithelia, Mixed-Breed_Canis familiarisCEA protein
Beef_Bos taurusHuman PSMA/FOLH1/NAALADase 1
Shrimp_Penacidae
Peanut_Arachis hypogaeaOther Antigens
Wheat, Whole_Triticum aestivumMyosin Light Chain
Mite, House Dust_Blomia tropicalisMuscarinic receptor
Bermuda_Cynodon dactylonAlbumin Bovine fraction V
Cedar,Red_Juniper rus virginianaHuman AQP4
Plantain, English_Plantago lanceolataDNA Polymerase beta Protein
Honey Bee_Apis melliferaEBV EBNA1
AGTR1(angiotension receptor1)

The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.

In-Campus Chip Price : $500.00  (contains 15 samples, price may vary for off-campus customers)
In Stock

Please select services from the following list:

In-Campus-Price Product
$50.00 Autoantibody Profiling Service for IgG and IgM
The whole process for profiling IgG and IgM autoantibodies from serum or other body fluids using our 98-plex and 128-plex autoantigen arrays. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be detected with Cy3 labeled anti-IgG and cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4400A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will be Excel spreadsheet containing Non-normalized and Normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $50 per sample (chip not included), maximum 15 samples per chip.
$75.00 Autoantibody Profiling Service for IgA and IgE
IgA and IgE are important autoantibody subtypes for many autoimmune, infection and allergic diseases. With our high-thourghput autoantigen microarray platform, we are able to detect autoreactive IgA and IgE autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this service, we can simultaneously detect autoreactive IgA and IgE autoantibodies against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgA and IgE secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgA or IgE). The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $75 per sample (chip not included), maximum 15 samples per chip.
$75.00 Autoantibody Profiling Service for IgG subclass IgG1 and IgG3
IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG1 and IgG3 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks.
$75.00 Autoantibody Profiling Service for IgG subclass IgG2 and IgG4
IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG2 and IgG4 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks.
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