Microarray Core Facility

Autoantigen Microarray Super Panel (128 antigen panel)

Autoantigen array super panel I contains 128 autoantigens and various internal controls. The antuantigens in this panel include most of nuclear antigens, cytoplasmic antigens, membrane antigen, phospholipid antigens, as well as some novel autoantigen identified from serum and tissues. The antigens in the Super Panel are listed below:

AggrecanssRNALa/SSBRo/SSA(60KDa)
alpha Fodrin(Sptan1) dsDNA Laminin S100
alpha-actinin EBNA1 LC1 Scl-70
Amyloid Elastin LKM1 Sm
AQP4 recombinant Entaktin EDTA M2 antigen Sm/RNP
BP1 Factor I Matrigel SmD
C1q Factor P MDA5 SmD1
Cardolipin Factor B Mi-2 SmD2
CENP-A Factor D Mitochondrial antigen SmD3
CENP-B Factor H MPO SP100
Chondroitin Sulfate C Fibrinogen IV Muscarinic receptor Sphingomyelin
Chromatin  Fibrinogen S Myelin basic protein (MBP) SPR54
Collagen I Fibronectin Myelin-associated glycoprotein-FC ssDNA
Collagen II GBM (disso) Myosin T1F1 GAMMACollagen
Collagen III Genomic DNA Nucleolin Thyroglobulin
Collagen IV Gliadin (IgG) Nucleosome antigen TNFa
Collagen V Glycated Albumin Nup62 Topoisomerase I
Collagen VI GP2 PCNA TPO
complement C1q gP210 Peroxiredoxin 1 TTG
complement C3 Histone H1 Phophatidylinositol U1-snRNP-68
complement C3a Histone H2A PL-12 U1-snRNP-A
complement C3b Histone H2B PL-7 U1-snRNP-BB'
complement C4 Histone H3 PM/Scl-100 U1-snRNP-C
complement C5 Histone H4 PM/Scl-75 Vimentin
complement C6 Hemocyanin POLB Vitronectin
complement C7 Heparan HSPG PR3 β2-glycoprotein I
complement C8 Heparin Proteoglycan β2-microglobulin
complement C9 Heperan Sulfate Prothrombin protien IgA - human and mouse
CPR antigen(human) Histone (total) Ribo phasphoprotein P1 IgE- human
Cytochrome C Intrinsic Factor Ribo phasphoprotein P2 IgG - human and mouse
Decorin-bovine Jo-1 Ribo phasphoprotein P0 IgM - human and mouse
DGPS KU (P70/P80) Ro/SSA (52KDa) Anti-IgG, IgA and anti-IgM

The autoantigens are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control.

In-Campus Chip Price : $750.00  (contains 15 samples, price may vary for off-campus customers)
In Stock

Please select services from the following list:

In-Campus-Price Product
$50.00 Autoantibody Profiling Service for IgG and IgM
The whole process for profiling IgG and IgM autoantibodies from serum or other body fluids using our 98-plex and 128-plex autoantigen arrays. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be detected with Cy3 labeled anti-IgG and cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4400A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will be Excel spreadsheet containing Non-normalized and Normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $50 per sample (chip not included), maximum 15 samples per chip.
$75.00 Autoantibody Profiling Service for IgA and IgE
IgA and IgE are important autoantibody subtypes for many autoimmune, infection and allergic diseases. With our high-thourghput autoantigen microarray platform, we are able to detect autoreactive IgA and IgE autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this service, we can simultaneously detect autoreactive IgA and IgE autoantibodies against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgA and IgE secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgA or IgE). The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $75 per sample (chip not included), maximum 15 samples per chip.
$75.00 Autoantibody Profiling Service for IgG subclass IgG1 and IgG3
IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG1 and IgG3 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks.
$75.00 Autoantibody Profiling Service for IgG subclass IgG2 and IgG4
IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG2 and IgG4 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks.
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