COVID-19 and other human virus antigen array
The ongoing coronavirus (COVID-19) pandemic highlights the importance of technologies aimed at anti-viral antibody detection. The Microarray (Immune Profiling) core at UT Southwestern has designed protein arrays that encompass 30 different virus species including SARS-CoV2 (Spike and Nucleocapsid Protein). The array can detect two types of antibodies (IgG, IgM, IgA, or IgG subclasses) simultaneously, and should be useful for both basic and clinical studies of COVID-19. The core is now ready to provide virus antibody profiling service to UT Southwestern clinical and basic science investigators. For details, please contact with Dr. Quan Li at quan.li@utsouthwestern.edu. The complete virus as list below:
| Adnovirus | Coronavirus (CoV) | Coxsackieviruses |
| Cytomegalovirus (CMV) | Dengus Virus | EB virus (EBV) |
| Echovirus | Enterovirus | Hepatitis A virus (VAV) |
| Hepatitis B virus (VBV) | Herpes Simplex virus (HSV) | Human coronavirus (HCoV-229E) |
| Human coronavirus (HCoV-NL63) | Human coronavirus (HCoV-OC43) | Human coronavirus HKU1 |
| Human MERS-CoV | Human SARS CoV | Human SARS CoV-2 (COVID-19) |
| Influenza A virus (Flu A) | Influenza B virus (Flu B) | Molluscum contagiosum virus (MCV) |
| Mumps Virus | Papillomavirus (HPV) | Parvovirus |
| Respiratory Syncytial Virus (RSV) | Rotavirus | Robella virus |
| Varicella Zoster Virus (VZV) | West Nile Virus |
The protein array are printed on 16-pad FAST slide. Each chip contains 16 identical arrays and can process 15 samples and one PBS control. In-Campus Chip Price : $750.00 (contains 15 samples, price may vary for off-campus customers)
In Stock
Please select services from the following list:
| In-Campus-Price | Product |
|---|---|
| $50.00 | Autoantibody Profiling Service for IgG and IgM The whole process for profiling IgG and IgM autoantibodies from serum or other body fluids using our 98-plex and 128-plex autoantigen arrays. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be detected with Cy3 labeled anti-IgG and cy5 labeled anti-IgM and the arrays will be scanned with GenePix® 4400A Microarray Scanner. The images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgG or IgM). The final data output will be Excel spreadsheet containing Non-normalized and Normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $50 per sample (chip not included), maximum 15 samples per chip. |
| $75.00 | Autoantibody Profiling Service for IgA and IgE IgA and IgE are important autoantibody subtypes for many autoimmune, infection and allergic diseases. With our high-thourghput autoantigen microarray platform, we are able to detect autoreactive IgA and IgE autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this service, we can simultaneously detect autoreactive IgA and IgE autoantibodies against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgA and IgE secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls (IgA or IgE). The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. $75 per sample (chip not included), maximum 15 samples per chip. |
| $75.00 | Autoantibody Profiling Service for IgG subclass IgG1 and IgG3 IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG1 and IgG3 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. |
| $75.00 | Autoantibody Profiling Service for IgG subclass IgG2 and IgG4 IgG autoantibody has 4 subclasses (IgG1, IgG2, IgG3 and IgG4) and each of them may associated with different clinical manifestations during the disease development. With our high-thourghput autoantigen microarray platform, we are able to detect subclasses of IgG autoantibodies to a broad range of autoantigens with high sensitivity and specificity. With this assay, we can simultaneously detect IgG1 and igG3 subclassed against up to 125 antigens on each array. Minimum 10ul of serum is required for each sample and each chip can process up to 15 samples. The samples will be treated with DNAse I, diluted 1:50, and incubated with autoantigen array. The autoantibodies binding to the antigens on the array will be assayed with Biotinylated and IgG2 and IgG4 secondary antibody and then detected with fluorescent labeled anti-biotin for imaging. The arrays will be scanned with GenePix® 4400A Microarray Scanner and images will be analyzed using GenePix 7.0 software to generate GPR files. The averaged net fluorescent intensity (NFI) of each autoantigen will be normalized to internal controls. The final data output will be Excel spreadsheet containing both non-normalized and normalized signal intensity as well as the Signal to Noise Ratio (SNR) for all antigens. The total turnaround time for sample processing will be around 2 weeks. |