E. coli Genome 2.0 Array
The GeneChip E. coli Genome 2.0 Array includes approximately 10,000 probe sets for all 20,366 genes present in four strains of E. coli. Due to the high degree of similarity between the E. coli strains, whenever possible, a single probe set is tiled to represent the equivalent ortholog in all four strains. The GeneChip E. coli Genome 2.0 Array tiles probe sets over the entire open reading frame (ORF) of E. coli, includes over 700 intergenic regions, probes for various antibiotic resistance markers, and incorporates additional control and reporter genes from the previous generation E. coli array.
Please select services from the following list:
|$500.00||Affymetrix Array Two-round Amplification Full Service
Unit price per sample. This service start from small amount of total RNA.
|$200.00||Affymetrix cRNA Probe Labeling
cRNA probe labeling only.
|$250.00||Affymetrix gene expression array full serivce from total RNA
Unit price per sample. This service includes cDNA synthesis, cDNA purification, QC of cDNA, aRNA synthesis, aRNA purification, hybridization, wash, staining, and scanning of slides.
|$100.00||Affymetrix gene expression array hybridization from cRNA
Unit price per sample. This service includes the fragmentation of biotin labelled cRNA immediately followed by an overnight hybridization onto an array. It also includes the washing, staining, and scanning of an array.
|$40.00||Affymetrix GX Chip Scanning
Affymetrix chip scanning only.
|$40.00||RNA/DNA Quality Check --Bioanalyzer Chip
$40 per chip, can process up to 12 samples if samples are in the range of 50-500 ng/ul (Nano Bioanalyzer Chip). Samples below 50 ng/ul will use Pico Bioanalyzer chip which holds 11 samples”. You must also order the service to complete this order
|$5.00||Sample Quality Check Using Bioanalyzer Service
$5 per sample. Please submit RNA samples in the concentration range of 15 to 500 ng/ul for use with the Nano chip. Samples for microarray should be in the nanogram range for concentration. Bioanalyzer only orders with RNA samples below 15 ng/ul may use the Pico chip. An RNA Integrity Number (RIN) greater than 8.0 should be sufficient for microarray experiment. The starting tissue or cells should be freshly harvested and immediately frozen (snap frozen) in liquid nitrogen. Keep the samples on dry ice or liquid nitrogen before submitting to prevent thawing. Freshly dissected tissues may also be kept in Ambion RNAlater solution at RT or 4C. You must order a Bioanalyzer chip also to complete your order!
|$50.00||Total RNA Extraction
Trizol and Qiagen RNeasy kit have been used to extract high-quality of total RNA. This service includes quality and quantity check.