Microarray Core Facility

Human Genome U133 Plus 2.0

All probe sets represented on the GeneChip Human Genome U133 Set are identically replicated on the GeneChip Human Genome U133 Plus 2.0 Array. The sequences from which these probe sets were derived were selected from GenBankĀ®, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

In addition, there are 9,921 new probe sets representing approximately 6,500 new genes. These gene sequences were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from the UniGene database (Build 159, January 25, 2003) and refined by analysis and comparison with a number of other publicly available databases, including the Washington University EST trace repository and the NCBI human genome assembly (Build 31).

HG-U133 Plus 2 Annotation

In-Campus Chip Price : $435.00  (price may vary for off-campus customers)
In Stock

Please select services from the following list:

In-Campus-Price Product
$500.00 Affymetrix Array Two-round Amplification Full Service
Unit price per sample. This service start from small amount of total RNA.
$200.00 Affymetrix cRNA Probe Labeling
cRNA probe labeling only.
$300.00 Affymetrix gene expression array full serivce from total RNA
Unit price per sample. This service includes cDNA synthesis, cDNA purification, QC of cDNA, aRNA synthesis, aRNA purification, hybridization, wash, staining, and scanning of slides.
$100.00 Affymetrix gene expression array hybridization from cRNA
Unit price per sample. This service includes the fragmentation of biotin labelled cRNA immediately followed by an overnight hybridization onto an array. It also includes the washing, staining, and scanning of an array.
$40.00 Affymetrix GX Chip Scanning
Affymetrix chip scanning only.
$40.00 RNA/DNA Quality Check --Bioanalyzer Chip
$40 per chip, can process up to 12 samples if samples are in the range of 50-500 ng/ul (Nano Bioanalyzer Chip). Samples below 50 ng/ul will use Pico Bioanalyzer chip which holds 11 samplesā€. You must also order the service to complete this order
$5.00 Sample Quality Check Using Bioanalyzer Service
$5 per sample. Please submit RNA samples in the concentration range of 15 to 500 ng/ul for use with the Nano chip. Samples for microarray should be in the nanogram range for concentration. Bioanalyzer only orders with RNA samples below 15 ng/ul may use the Pico chip. An RNA Integrity Number (RIN) greater than 8.0 should be sufficient for microarray experiment. The starting tissue or cells should be freshly harvested and immediately frozen (snap frozen) in liquid nitrogen. Keep the samples on dry ice or liquid nitrogen before submitting to prevent thawing. Freshly dissected tissues may also be kept in Ambion RNAlater solution at RT or 4C. You must order a Bioanalyzer chip also to complete your order!
$50.00 Total RNA Extraction
Trizol and Qiagen RNeasy kit have been used to extract high-quality of total RNA. This service includes quality and quantity check.
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